Journal: RNA

Article Title: Comparison of CpG- and UpA-mediated restriction of RNA virus replication in mammalian and avian cells and investigation of potential ZAP-mediated shaping of host transcriptome compositions

doi: 10.1261/rna.079102.122

Figure Lengend Snippet: Replication of E7 replicons in control A549 and chicken DF-1 cell line. Cells were transfected with 50 ng/well of in vitro transcribed RNA of E7 replicon with a 3′UTR variant WT, permuted (CDLR), CpG low (cu) and elevated of CpG (CpG-H) and UpA (UpA-H) and assayed for luciferase expression at 6 h post transfection. Relative replication in control human cell lines A549, A549 ZAP k/o, and DF-1 cells. Significance of differences from WT replication were calculated by two-tailed paired t -test; asterisks show significance values as follows: (***) P < 0.001, (**) P < 0.01, and (*) P < 0.05

Article Snippet: Human A549 (purchased from ATCC), A549 ZAP −/−/− (CRISPR engineered from WT A549 cells), chicken DF-1, Hek293T, MDBK, zzR-127, BFA, FBT, AK-D, and the primary cell lines for pigeon, partridge, and pheasant were maintained in DMEM- Ham (F12) media supplemented with 10% FCS, 2 mM glutamine, 1% nonessential amino acids, and 1% P/S.

Techniques: Control, Transfection, In Vitro, Variant Assay, Luciferase, Expressing, Two Tailed Test

Journal: RNA

Article Title: Comparison of CpG- and UpA-mediated restriction of RNA virus replication in mammalian and avian cells and investigation of potential ZAP-mediated shaping of host transcriptome compositions

doi: 10.1261/rna.079102.122

Figure Lengend Snippet: Comparison of the replication kinetics of WT IAV with mutants with elevated ( A ) CpG and ( B ) UpA dinucleotide frequencies in segment 4. Replication was assayed in mutants in the avian cell lines QT6 (Japanese Quail fibrosarcoma— Coturnix japonica ), DF-1 (chicken embryo fibroblast— Gallus gallus ) and CCL-141 (duck embryo— Anas platyrhynchos ). Cells were infected at an MOI of 0.001, and at specified times supernatant was collected and titrated by infectivity using A549 ZAP k/o cells—TCID 50 values shown on the y -axis. Data points represent the mean of two biological replicates; the reduced replication in the replication of the CpG-H compared to WT was significant in unpaired value t -test at 24 h and 36 h time points, (*) P = 0.03, (**) P = 0.04, and by two-way ANOVA ( P = 0.022). No other comparisons of mutant (CpG-H or UpA-H) or time point with WT were significant at the P = 0.05 level.

Article Snippet: Human A549 (purchased from ATCC), A549 ZAP −/−/− (CRISPR engineered from WT A549 cells), chicken DF-1, Hek293T, MDBK, zzR-127, BFA, FBT, AK-D, and the primary cell lines for pigeon, partridge, and pheasant were maintained in DMEM- Ham (F12) media supplemented with 10% FCS, 2 mM glutamine, 1% nonessential amino acids, and 1% P/S.

Techniques: Comparison, Infection, Mutagenesis

Journal: Annual review of biomedical engineering

Article Title: Cell and Tissue Therapy for the Treatment of Chronic Liver Disease

doi: 10.1146/annurev-bioeng-112619-044026

Figure Lengend Snippet: Liver tissue engineering approaches. Hepatic tissue engineering consists of cellular components and engineered 3D scaffolds that are assembled and transplanted in vivo to yield hepatic function.

Article Snippet: A biomimetic in vitro liver tissue engineering model will ideally include several cell types that partake in the fibrogenic cascade such as hepatocytes, hepatic stellate cells, LSECs, and immune cells.

Techniques: In Vivo

Journal: Advanced healthcare materials

Article Title: In Vitro Tissue-Engineered Skeletal Muscle Models for Studying Muscle Physiology and Disease

doi: 10.1002/adhm.201701498

Figure Lengend Snippet: Methods for the in vitro engineering of 3D muscle tissues. Schematics showing A) scaffold and B) scaffold-free methods for engineering skeletal muscle. Abbreviations: SIS, small intestine submucosa; PIPAAm, poly(N-isopropylacrylamide); and PDMS, polydimethylsiloxane.

Article Snippet: Thus, the ability to engineer a biomimetic 3D muscle environment in vitro could overcome several limitations of traditional 2D cell culture systems.

Techniques: In Vitro

Journal: Advanced healthcare materials

Article Title: In Vitro Tissue-Engineered Skeletal Muscle Models for Studying Muscle Physiology and Disease

doi: 10.1002/adhm.201701498

Figure Lengend Snippet: Summary of disease models using human muscle cells. Compilation of published works using human cells to study muscle diseases listed in chronological order. For each study, key parameters including cell type (iPSC-MyoD indicates MyoD overexpression; iPSC-SMD indicates small molecule differentiation; and primary cells), culture method, disease modeled (LGMD2B, Limb girdle muscular dystrophy 2B; CPT-II, carnitine palmitoyltransferase II; DMD, Duchenne muscular dystrophy; BMD, Becker muscular dystrophy), reported phenotype (CK, creatine kinase; GAA, acid alpha-glucosidase), treatment or correction tested (AON, antisense oligonucleotides; HAC, human artificial chromosome; TFEB, transcription factor EB), and observed therapeutic effects have been shown.

Article Snippet: Thus, the ability to engineer a biomimetic 3D muscle environment in vitro could overcome several limitations of traditional 2D cell culture systems.

Techniques: Over Expression, HAC Assay, Membrane, Expressing, Control, CRISPR, Knock-In, Mutagenesis, Inhibition, Activity Assay, Recombinant